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KMID : 0382619900100010470
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Hanyang Journal of Medicine
1990 Volume.10 No. 1 p.470 ~ p.484
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Stability of R-plasmid in Beta-lactamase Producing Neisserau gonorrhoeal
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梁仁愛/Yang, In Sub
金永泰/金在烘/高在炅/金重煥/Kim, Young Tae/Kim, Jae Hong/Koh, Jai Kyung/Kim, Joong Hwan
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Abstract
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Since its first isolation, penicillinase producing Neisseria gonorrhoeae (PPNG) has spread throughout the world and has become rampant in Korea, where its prevalence was over 50% in 1987.
One of the most important factors in evaluating the possible spread of a bacterial R-plasmid is its stability. In this study, the stability of R-plasmid in PPNG was evaluated.
The stability of the R-plasmid in 7 PPNG strains was determined, Strains were passaged every other day on chocolate agar for 3 weeks and on chocolate agar with 2㎍ of penicillin G per ml over 8 weeks. Then, all individual clones were tested with the chromogenic cephalosporin and representative clones were examined by agarosegel electrophoresis.
Under passage on media without the antibiotic, plasmid loss was noted on 2 PPNG strains lacking the conjugative 25-megadalton (Md) plasmid (PPNG 69F,PPNG 413). Their plasmid loss were 1.7% and 17.2%, respectively. However, plasmid loss was not noted on the remaining 5 PPNG strains carrying the conjugative plasmid. Under passage on the antibiotic containing media, no plasmid loss was noted in and strain.
The stability of the gonococcal R-plasmid in E. coli transformats, YMC-10 (pNG 69F) and YMC-10 (pNG 132) was determined. Strains were passaged every other day on Muller-Hinton agar and on Muller-Hinton agar with 30, 125, 250 and 500㎍ of ampicillin per ml for 3 weeks. Then all individual clones were examined for betalactamase production and representative clones were examined by agarose gel electrophoresis.
The plasmid loss was not noted in any strain.
It is suggested that the R-plasmids of circulating PPNG in Korea are stable and the steep increase of PPNG prevalence in Seoul may be correlated with this high stability of the R-plasmid.
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KEYWORD
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